Ryzen 4500 and Ryzen 5500 only support PCIe 3.0 as well.
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The RX 6600 only uses PCIe 4.0 X8 so there could be performance loss if only in PCIe 3.0 mode.
The requirements I have read say you can run SAM in both PCIe 3.0 and 4.0.
Ryzen 7 7800X3D, ASUS ROG STRIX B650E-F GAMING WIFI, G.SKILL TRIDENT Z NEO 2x16GB DDR5 DRAM 6000MT/s CL30, AMD Radeon RX 7900 XTX, LIAN LI EDGE 1300, Corsair MP600 PRO NH 4TB